Research technologist III

at Ann & Robert H. Lurie Children's Hospital of Chica in Lake Forest, Illinois, United States

Job Description

Ann & Robert H. Lurie Children's Hospital of Chicago provides superior pediatric care in a setting that offers the latest benefits and innovations in medical technology, research and family-friendly design. As the largest pediatric provider in the region with a 140-year legacy of excellence, kids and their families are at the center of all we do. Ann & Robert H. Lurie Children's Hospital of Chicago is ranked in all 10 specialties by the U.S. News & World Report.

LocationSimpson Querrey Biomedical Research Center

Job Description
General Summary:
Seeking a highly motivated candidate to fill a full-time position as a research technician in Dr. Loretta Li's laboratory at Lurie Children's Hospital / Northwestern University. We are a recently established translational leukemia research laboratory that uses a variety of biochemical, molecular, and cell biology approaches to identify new therapeutic targets, develop preclinical models of disease, and test novel therapeutics both in vitro and in vivo using mouse models. The research technician would work on a project studying acute lymphoblastic leukemia (ALL). The successful candidate will be a team player capable of handling multiple tasks and adaptable to a fast-paced research environment.

Essential Job Functions:
The research technician will conduct experiments to advance the research progress in the lab. The research technician will also assist with tasks to ensure the smooth day-to-day operation of the lab. The successful candidate will be responsible for the following:

Operation and maintenance of standard lab equipment.

Maintenance of cell lines in culture and testing novel therapeutics using in vitro proliferation assays.

Preparation of reagents and media used in lab experiments.

Basic handling of DNA and RNA including sample isolation, PCR, and preparation of samples for sequencing.

Protein isolation from cells and primary murine samples, Western blotting, and immunoprecipitation experiments.

Multi-color flow cytometry.

Transfection and retroviral / lentiviral transduction of murine and human cells for overexpression experiments or CRISPR-Cas9 knockout experiments.

Possible CRISPR guide RNA design and molecular cloning.

Possible testing of novel therapeutics in mouse models of leukemia. Previous experience working with mice is preferred.

Shared responsibility with other lab members for lab maintenance including possible ordering and stocking of laboratory supplies and maintenance of lab inventory.

Maintaining detailed records of their laboratory work, results, and data analyses.

1. Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 3 years' lab/bench or other relevant experience required; OR Successful completion of a full course of study in an accredited college or university leading to a master's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 1 year lab/bench or other relevant experience.
2. Working knowledge of computers, ability to troubleshoot, work independently, and function as a team member is essential. Therefore, experience in regular interaction with outside groups is highly desirable.
3. Excellent command of the English language and the ability to communicate effectively.
4. Strong organizational skills including the ability to establish priorities, react promptly to a large variety of requests, and the ability to keep the workplace clean and organized.
5. Previous lab/bench research experience and familiarity with most of the techniques listed above highly preferred.
6. Experience handling mice is preferred. This includes experience with breeding and weaning the animals, bleeding the mice, tail vein injections, IV, IP, and PO dosing, and necropsy.
7. The candidate with flexibility in their schedule to work on weekends and some evenings as needed to meet project goals is highly preferred.

EducationBachelor's Degree: Biomedical Science (Required)

Benefit Statement

For full time and part time employees who work 20 or more hours per week we offer a generous benefits package that includes:
Medical, dental and vision insurance
Employer paid group term life and disability
Employer contribution toward Health Savings Account
Flexible Spending Accounts
Paid Time Off (PTO), Paid Holidays and Paid Parental Leave
403(b) with a 5% employer match

Various voluntary benefits:

Supplemental Life, AD&D and Disability

Critical Illness, Accident and Hospital Indemnity coverage

Tuition assistance

Student loan servicing and support

Adoption benefits

Backup Childcare and Eldercare

Employee Assistance Program, and other specialized... For full info follow application link.

EEO/AA Employer/Vets/Disability

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Job Posting: 10970508

Posted On: May 29, 2023

Updated On: Jun 16, 2023

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