at Rosalind Franklin Univeristy of Medicine & Science in North Chicago, Illinois, United States
Job Description
Position Summary
Rosalind Franklin University of Medicine and Science, College of Pharmacy, invites applications for a full-time biology/biochemistry Research Technician. The Research technician will procure and culture various cell lines, and evaluate the effects of drugs on the cells including anticancer effects. In addition, the successful candidate will clone recombinant genes and perform transfections and analyze gene expression in cells for compound screening in our drug discovery programs. The successful applicant should also be able to establish and run cell-based and biochemical assays including radioisotope and fluorescence-based micro-titer plate assays. The individual will also work in core facilities to run flow cytometry and confocal microscopy assays. This work will take place under the supervision of Dr. John K. Buolamwini, Department of Pharmaceutical Sciences in the RFUMS College of Pharmacy. Responsibilities will include procuring and maintaining mammalian cell cultures, cell counting and set up of cells in quantitative numbers for experiments. Plan experiments with the supervisor and execute the experiments, maintain experimental notebooks, and provide reports to the supervisor. The individual will also provide limited assistance to student interns, or rotation students to do their experiments.
Our generous benefits package includes medical, dental, and vision plans; paid parental leave; short term and long term disability plans; life insurance; flexible spending accounts; and a 403(b) retirement plan which includes a 10% employer contribution after 2 years of service. RFUMS is committed to employee wellbeing and work-life balance. Full time staff are eligible for 3 weeks of vacation, 15 sick days, and 13 holidays plus two floating holidays. That is a minimum of 30 paid days off each year!
Who We Are
Rosalind Franklin University of Medicine and Science (RFU) is a graduate health sciences university committed to serving the population through the interprofessional education of health and biomedical professionals and the discovery of knowledge dedicated to improving wellness. The university embodies the spirit of inquiry and excellence modeled by its namesake Dr. Rosalind Franklin, whose Photo 51 was crucial to solving the structure of DNA.
Today, the university's 6 colleges continue Dr Franklin's legacy through rigorous academics, pioneering research, and innovative community service. Our unique interprofessional education model allows students to learn from and with each other in a variety of settings, including our simulation labs, the student-led Interprofessional Community Clinic, and through partnerships with area hospitals and clinics. RFU is a national leader in interprofessional research and practices, and is nationally recognized for its research in areas including neuroscience, brain-related diseases, inherited disorders, proteomics, cancer cell biology and immunology, cardiac resuscitation, and gait and balance.
Essential Duties & Responsibilities
* Help to maintain biology research laboratory;
* Procure and propagate mammalian cell lines;
* Perform gene transfections for transient and stable gene expression in cell lines;
* Protein expression and purification;
* Make plasmid preps, subclone genes and make new gene constructs;
* Perform site-directed mutagenesis of genes;
* Perform Western blotting, immunoprecipitation, and ELISA;
* Perform RT-PCR real-time PCR analysis;
* Prepare and purify RNA samples for gene expression and microarray microRNAs analyses;
* Perform siRNAs, shRNA, gene knockdown transfections;
* Perform anticancer drug testing in cell culture;
* Perform cell-based solute transporter assays;
* Perform live mitochondria isolation and analysis;
* Perform flow cytometry analysis.
Conditions of Employment
Must achieve satisfactory results from a background check
Must have proof of COVID 19 vaccination with booster or a university approved exemption
Required Education & Experience
BS in molecular biology, biotechnology or equivalent with two years of job experience from a properly accredited college or university;
At least two years of experience performing similar work.
Required Knowledge, Skills, & Abilities
Possess excellent interpersonal communication skills;
Commitment to and ability to embrace diversity;
Must have strong work ethic and high level of integrity;
Computer literate; proficient in Microsoft Office Suite;
Possess excellent skills in verbal, written, telephone and email communications.
Preferred Qualifications
Experience with cell culture, performing and establishing biological assays, particularly cell-based assays.
Experience with running Flow cytometry experiments with... For full info follow application link.
Rosalind Franklin University of Medicine & Science is an Equal Opportunity Employer of Minorities, Females, Protected Veterans, and Individuals with Disabilities. EOE, Including Disability / Vets.
