at Northwestern University in Evanston, Illinois, United States
Job Description
Job Opening Id: 49901
Department: MED-Cell&DevelopmentalBiology
Salary/Grade: NEX/13
Job Summary:
Participates in design and execution of research lab projects. Conducts experimental tests and procedures and processes and analyzes data. Reviews and revises existing research methodologies and may develop new methodologies, mathematical and/or computer models for predicting data. May ensure overall lab compliance with all associated rules and regulations.
Specific Responsibilities:
- Following overall research objectives, independently designs, plans, conducts and troubleshoots experiments.
- Evaluates protocols and data obtained from groups of experiments and recommends changes to processes, procedures and techniques to improve quality and validity of results based upon overall project objectives.
Molecular Biology:
- DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting
- PCR, RT-PCR
- Plasmid preparation
- Cloning, mapping and sequencing of DNA Recombinant virus production and purification
Cellular Biology:
- Cell culture, cell isolation Functional studies Transfection
Histology:
- Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining Immunohistochemistry
- Microscopy
Immunology:
- Flow cytometry ELISA, Elispot assay Cytokine assay
- Antibody production and purification
