at AbbVie US LLC in North Chicago, Illinois, United States
Job Description
Sr. Scientist I - Cell & Molecular Biologist, AbbVie Inc., North Chicago, IL. Design, execute, & interpret critical experiments to answer scientific questions. Generate & test hypothesis by designing, performing, & interpreting laboratory-based experiments. Read literature critically & analyze genomic data to support these activities. Develop in vitro & in vivo screening assays in collaboration with therapeutic area scientists by generating cell lines, developing lentiviral & RNP delivery methods, & integrating & optimizing screening protocols. Responsible for transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, & performing CRISPR screens in neuronal & glial cell types. Perform pooled & arrayed CRISPR screens with FACS-based molecular & functional assays on immortalized cell lines, primary cells, & iPSC-derived cells. Work closely with NGS & bioinformatics scientists to process genomic samples, analyze screening results, & generate screening hits for follow-up studies. Investigate novel screening hits in the context of disease biology by generating & testing hypothesis using a variety of biochemical, molecular, cellular, & genomic assays. Utilize molecular analytic methods including DNA/RNA isolation, PCR/qPCR, molecular cloning, genotyping, &/or western blot. Map candidate genes to known disease genetic networks through experimental & bioinformatic approaches. Learn, understand, & master new experimental techniques, & act as a resource or mentor for others. Perform RNA-sequencing, RNAi (shRNA/siRNA), & CRISPR gene editing. Collaborate and communicate with cross-functional scientists on experimental designs and results and scientific goals. Report research progress to management on a regular basis, provide project updates to cross-functional project teams, and provide scientific presentations to department and other internal scientific forums. Seek and advance opportunities to present research results at external scientific conferences & publish high-impact articles in peer-reviewed journals. Must possess a PhD or foreign academic equivalent in Biochemistry and Molecular Biology, Biological Sciences, Chemistry, or a closely related scientific field with an academic or industrial background in: (i) transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, & performing CRISPR screens in neuronal & glial cell types; (ii) molecular analytic methods including DNA/RNA isolation, PCR/qPCR, molecular cloning, genotyping, &/or western blot; & (iii)RNA-sequencing, RNAi (shRNA/siRNA), & CRISPR gene editing. Apply online at https://careers.abbvie.com/en & reference REF23197A
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