at Northwestern University in Evanston, Illinois, United States
Job Description
Job Opening Id: 53905
Department: MED-Allergy
Salary/Grade: NEX/11
Job Summary:
Conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s). May complete mathematical calculations, and/or computer tasks and/or assist in judging validity of data. Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices.
Specific Responsibilities:
- Following protocols, designs, sets-up and conducts basic experiments.
- Analyzes and troubleshoots protocols and data obtained from individual experiments and recommends alternatives and suggestions to improve processes, validity and/or accuracy.
- Using a fully functioning level of skill, completes the following activities:
Molecular Biology:
- DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting
- PCR, RT-PCR
- Plasmid preparation
- Cloning, mapping and sequencing of DNA
- Recombinant virus production and purification
Cellular Biology:
- Cell culture, cell isolation
- Functional studies
- Transfection
Histology:
- Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
- Immunohistochemistry
- Microscopy
Immunology:
- Flow cytometry
- ELISA, Elispot assay
- Cytokine assay
- Antibody production and purification
