at Northwestern University in Evanston, Illinois, United States
Job Description
Job Opening Id: 54096
Department: MED-Pulmonary
Salary/Grade: NEX/15
Job Summary:
Conducts complex experimental research in a research lab, functioning as a specialist or technical expert. May manage one or more phase(s) of a major project(s) or all activities of a small project. May ensure overall lab compliance with all associated rules and regulations.
Specific Responsibilities:
- Acting as a technical expert or specialist in a particular field within a research lab, establishes and optimizes protocols and coordinates conduct of multiple complex experiments.
- Reviews results and revises protocols as necessary to ensure that project objectives are attained.
- Using an expert level of skill, completes the following activities:
Molecular Biology:
- DNA, RNA, Protein isolation, purification and expression
- Southern, Northern, Western blotting
- PCR, RT-PCR
- Plasmid preparation
- Cloning, mapping and sequencing of DNA
-
Recombinant virus production and purification
Cellular Biology:
- Cell culture, cell isolation
- Functional studies
-
Transfection
Histology:
- Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
- Immunohistochemistry
- Microscopy
Immunology:
- Flow cytometry
- ELISA, Elispot assay
